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vegfc  (R&D Systems)


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    Structured Review

    R&D Systems vegfc
    Vegfc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf+c+cys156ser/Recombinant+Human+VEGF-C+(Cys156Ser)+Protein/pm41944190-231-16-17
    Average 94 stars, based on 41 article reviews
    vegfc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells.
    Article Snippet: Article Chronology: Received 26 November 2010 Revised version received 17 July 2011 Accepted 23 July 2011 Available online 2 August 2011 Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes.. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs).. LECs were successfully isolated from murine dermis and prostate.

    Article Title: Vascular Endothelial Growth Factor Receptor-3 Directly Interacts with Phosphatidylinositol 3-Kinase to Regulate Lymphangiogenesis
    Article Snippet: The other antibodies used were: anti-phospho PI3K p85 (Tyr458/p55 Tyr199; Cat. no. 4228; Millipore, MA) and total PI3K p85 (Cat. no. 06–19; Millipore); mouse anti-human CD31 (Clone: JC70A, Cat. no. M0823; DAKO, Denmark); mouse anti-human podoplanin (Clone: D2-40, Cat. no. 730-23; Signet Laboratories, MA); mouse anti-human VEGFR-2 (Cat. no. AF357; R&D Systems, MN), goat anti-human VEGFR-3 (Cat. no. AF349; R&D Systems); mouse anti-human pan-actin (Clone: Ab-5, NeoMarkers, CA); Alexa Fluor 488- and 568-conjugated secondary antibodies (Invitrogen, CA), and IRDye700DX conjugated anti-mouse IgG and IRDye800CW conjugated anti-rabbit IgG (Rockland Immunochemicals, PA). .. Recombinant human VEGF-A165 (Cat. no. 293-VE), VEGF-C (Cat. no. 2179-VC), VEGF-C Cys156Ser (Cat. no. 752-VC/CF), VEGF-D (Cat. no. 622-VD) and hepatocyte growth factor (HGF; Cat. no. 294-HG) were purchased from R&D Systems. .. LY294002 and GF109203X were purchased from Calbiochem (CA).

    Incubation:

    Article Title: Vascular endothelial growth factors A and C are induced in the SVZ following neonatal hypoxia-ischemia and exert different effects on neonatal glial progenitors
    Article Snippet: .. OPCs were then incubated with VEGF-C (Cys156Ser) (R&D Systems; 100ng/ml) and blocking antibodies to either VEGFR-1 (cat #AF471, R&D Systems, 5μg/ml), or VEGFR-2 (cat #AF644, R&D Systems, 0.2 μg/ml), or VEGFR-3 (ImClone IMC-3C5, 7.5μg/ml), or N2B2 medium control for 18 h. IMC3C5 is an antagonist antibody for human VEGFR-3 with cross-reactivity to rat VEGFR-3 (ImClone Systems, unpublished data). .. After treatment, the OPCs were fixed for 10 min in 3% PFA and then incubated with primary antibodies against Ki67 (Vector Laboratories, 1:1000) and A2B5 (supernatant, 1:5) or O4 (supernatant, 1:4) to identify the proliferating oligodendrocyte at both early and late stages, respectively, at 4°C overnight.

    Article Title: Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth
    Article Snippet: .. To analyse human LEC proliferation, cells were starved overnight in basal medium without growth factors (EBM‐2 MV, Promocell or Lonza), washed with PBS and incubated with 100 ng/ml VEGF‐C Cys156Ser (R&D Systems) and 10 μM 5‐bromo‐2′‐deoxyuridine (BrdU, Sigma) for 1 h. Cells were washed with PBS, immediately fixed in ethanol fixative (70% ethanol, 30% glycine 50 nM), and staining was performed with mouse anti‐BrdU antibody (BD Bioscience, 555627). .. Secondary antibody conjugated with AF488 (Molecular probes) was used, as was DAPI (Sigma) to counterstain cell nuclei.

    Blocking Assay:

    Article Title: Vascular endothelial growth factors A and C are induced in the SVZ following neonatal hypoxia-ischemia and exert different effects on neonatal glial progenitors
    Article Snippet: .. OPCs were then incubated with VEGF-C (Cys156Ser) (R&D Systems; 100ng/ml) and blocking antibodies to either VEGFR-1 (cat #AF471, R&D Systems, 5μg/ml), or VEGFR-2 (cat #AF644, R&D Systems, 0.2 μg/ml), or VEGFR-3 (ImClone IMC-3C5, 7.5μg/ml), or N2B2 medium control for 18 h. IMC3C5 is an antagonist antibody for human VEGFR-3 with cross-reactivity to rat VEGFR-3 (ImClone Systems, unpublished data). .. After treatment, the OPCs were fixed for 10 min in 3% PFA and then incubated with primary antibodies against Ki67 (Vector Laboratories, 1:1000) and A2B5 (supernatant, 1:5) or O4 (supernatant, 1:4) to identify the proliferating oligodendrocyte at both early and late stages, respectively, at 4°C overnight.

    Control:

    Article Title: Vascular endothelial growth factors A and C are induced in the SVZ following neonatal hypoxia-ischemia and exert different effects on neonatal glial progenitors
    Article Snippet: .. OPCs were then incubated with VEGF-C (Cys156Ser) (R&D Systems; 100ng/ml) and blocking antibodies to either VEGFR-1 (cat #AF471, R&D Systems, 5μg/ml), or VEGFR-2 (cat #AF644, R&D Systems, 0.2 μg/ml), or VEGFR-3 (ImClone IMC-3C5, 7.5μg/ml), or N2B2 medium control for 18 h. IMC3C5 is an antagonist antibody for human VEGFR-3 with cross-reactivity to rat VEGFR-3 (ImClone Systems, unpublished data). .. After treatment, the OPCs were fixed for 10 min in 3% PFA and then incubated with primary antibodies against Ki67 (Vector Laboratories, 1:1000) and A2B5 (supernatant, 1:5) or O4 (supernatant, 1:4) to identify the proliferating oligodendrocyte at both early and late stages, respectively, at 4°C overnight.

    Phospho-proteomics:

    Article Title: Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth
    Article Snippet: To determine VEGFR3 phosphorylation in mouse skin lysates, ears were collected and the inner ear layer was immediately homogenised in ice‐cold lysis buffer containing 50 mM HEPES pH 7.0, 150 mM NaCl, 10% glycerol, 1% Triton X‐100, 1 mM activated Na 3 VO 4 , phosphatase inhibitors (Phosphatase Inhibitor Cocktail, Roche or Sigma‐Aldrich) and protease inhibitors (cOmplete, Protease Inhibitor Cocktail Tablets, Roche). .. For analysis of human VEGFR3 phosphorylation, cells were starved overnight in basal medium without growth factors (EBM‐2 MV, Promocell or Lonza), washed with PBS and stimulated for 5 min with 100 ng/ml VEGF‐C Cys156Ser (R&D Systems). ..

    Article Title: Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth
    Article Snippet: .. Cells were authenticated by stimulation with 100 ng/ml VEGF‐C Cys156Ser (R&D Systems) and subsequent detection of VEGFR3 phosphorylation. ..

    Staining:

    Article Title: Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth
    Article Snippet: .. To analyse human LEC proliferation, cells were starved overnight in basal medium without growth factors (EBM‐2 MV, Promocell or Lonza), washed with PBS and incubated with 100 ng/ml VEGF‐C Cys156Ser (R&D Systems) and 10 μM 5‐bromo‐2′‐deoxyuridine (BrdU, Sigma) for 1 h. Cells were washed with PBS, immediately fixed in ethanol fixative (70% ethanol, 30% glycine 50 nM), and staining was performed with mouse anti‐BrdU antibody (BD Bioscience, 555627). .. Secondary antibody conjugated with AF488 (Molecular probes) was used, as was DAPI (Sigma) to counterstain cell nuclei.



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